Distribution of Bathyarchaeota (MCG) archaea in the South China Sea sediments and implication of its ecological roles

Abstract:

Tiantian Yu^1,2, Mingyang Niu^1,2 and Fengping Wang^1,2

 

¹State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai, 200240, China; ²State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China

ABSTRACT: Archaea of Bathyarchaeota Phylum (Miscellaneous Crenarchaeotal Group, MCG) are widespread and abundant in various marine and continental environments. However, very little is understood on the metabolisms and ecological roles of this cosmopolitan sedimentary archaea , partly due to obstacles of cultivation. Bathyarchaeota could be divided into large members of subgroups with potentially high genetic heterogeneity, implying different metabolic capabilities and functions in different subgroups. Here, we report our work on investigating Bathyarchaeota abundance and types of subgroups in the South China Sea(SCS) sediments, aim to understand their ecological roles in the carbon cycling. Bathyarchaeota were found the most dominant archaeal group in the sediments of SCS , most of which belong to subgroups MCG-8, MCG-15, MCG-17 and MCG-3. A new pair of 16S rRNA gene primers was designed as the published primers could not cover MCG-15 and MCG-17, which are the most dominant subgroups in the SCS sediments. Comparing with previous primers which used for quantifying the abundance of Bathyarchaeota via Q-PCR, the new primer revealed about an order of magnitude higher abundance in all South China Sea sediments. The relationship of geochemical parameters and MCG subgroups will further be explored to provide the basis for further understanding the metabolic function and biogeochemical roles of these poor-understood sedimentary archaea.