Defining Conserved Epibiotic Bacterial Genomes in the Trichodesmium Holobiont Using New Isolate Genomes and Field ‘Omic Techniques

Eric A Webb1, Yiming Zhao2, Noelle Held3, Elaina D Graham4, Asa Conover5, Jacob Semones5, Yuan Yuan Feng6, Feixue Fu7, Mak A Saito8, David A Hutchins7 and Michael D Lee1, (1)University of Southern California, Marine and Environmental Biology, Los Angeles, CA, United States, (2)University of Southern California, Department of Biological Sciences, Los Angeles, CA, United States, (3)Woods Hole Oceanographic Institution, Woods Hole, MA, United States, (4)University of Southern California, Marine & Environmental Biology, CA, United States, (5)University of Southern California, Marine and Environmental Biology Section, Los Angeles, CA, United States, (6)Tianjin University of Science and Technology, College of Marine and Environmental Sciences,, Tianjin, China, (7)University of Southern California, Los Angeles, CA, United States, (8)WHOI, Woods Hole, MA, United States
Abstract:
The colony-forming, nitrogen fixing cyanobacteriaTrichodesmium spp. are important sources of ‘new’ nitrogen in the oligotrophic ocean. Despite this central biogeochemical role, many questions about their evolution, physiology and trophic interactions remain unanswered. Leveraging the high depth and low cost of next generation sequencing and the USC Trichodesmium culture collection (USCTCC), we describe six new Trichodesmium enrichment genomes and 12 hand-picked colony metagenomes from the 2018 Trans-Atlantic TriCoLim R/V Atlantis cruise. Mapping enrichment genomes to metagenome reads demonstrated remarkable conservation between two closely related Trichodesmium thiebautii isolate genomes (one from the Pacific and the other from the Atlantic Oceans) and field Trichodesmium (recruiting up to 50% of the environmental sequences). These data indicate that USCTCC isolates are ‘representative’ of colonial Trichodesmium in situ. Furthermore, de novo assembly of associated bacterial genomes from metagenomes and enrichment cultures, show that epibionts are conserved both in laboratory and natural samples and are phylogenetically different from free-living bacteria. The biogeochemical implications and genomic evolution of these potential mutualisms will be discussed.