ME33A:
From Microbes to Mammals: Using Environmental DNA Sequencing to Advance Marine Biomonitoring and Management I

Session ID#: 93064

Session Description:
Environmental DNA sequencing represents an increasingly critical tool for conducting rapid biodiversity assessments across diverse marine habitats. Recent high-throughput sequencing studies have focused on the detection of unseen species (from microbes to mammals), baseline biodiversity assessments in critical ecosystems (e.g. Kelp Forests and lease areas for deep-sea mining), and tracking anthropogenic impacts related to activities such as aquaculture and oil spills. However, many challenges still remain for incorporating new “–Omics” methodologies (metabarcoding, metagenomics) into routine biomonitoring and environmental management projects, including recovery of false positive/negatives, taxonomic bias in eDNA sequencing datasets, and the lack of standardized reference databases and bioinformatics pipelines. This session will highlight new scientific insights and technological advances from recent environmental –Omics studies focused on marine biodiversity and biomonitoring. Importantly, we will highlight interdisciplinary approaches spanning a broad range of taxonomic groups (microbial, invertebrate, and vertebrate studies), and emphasize links between “traditional” biomonitoring surveys and cutting-edge –Omics techniques.
Co-Sponsor(s):
  • CP - Coastal and Estuarine Processes
Index Terms:
Primary Chair:  Holly Bik, University of Georgia, Department of Marine Sciences, Athens, GA, United States
Co-chairs:  Susanna Theroux1, Eric D Stein2 and David Gillett2, (1)Southern California Coastal Water Research Project, Costa Mesa, United States(2)Southern California Coastal Water Research Project, Costa Mesa, CA, United States
Primary Liaison:  Holly Bik, University of Georgia, Department of Marine Sciences, Athens, GA, United States
Moderators:  Holly Bik, University of Georgia, Department of Marine Sciences, Athens, GA, United States and Susanna Theroux, Southern California Coastal Water Research Project, Costa Mesa, United States
Student Paper Review Liaison:  Holly Bik, University of Georgia, Department of Marine Sciences, Athens, GA, United States

Abstracts Submitted to this Session:

Investigating Patterns of Larval Fish Community Dynamics Over the Past Two Decades Using a Novel Application of Environmental DNA Metabarcoding (637473)
Zachary Gold1, Dovi Kacev2, Paul H Barber3, Kelly D. Goodwin4, Luke Thompson5 and Andrew Thompson5, (1)University of California Los Angeles, Ecology and Evolutionary Biology, Los Angeles, CA, United States, (2)Scripps Institute of Oceanography, La Jolla, CA, United States, (3)University of Los Angeles, Ecology and Evolutionary Biology, Los Angeles, CA, United States, (4)NOAA Miami, Ocean Chemistry and Ecosystems Division, Atlantic Oceanographic and Meteorological Laboratory, Miami, FL, United States, (5)NOAA SWFSC, La Jolla, CA, United States
Metabarcoding from Microbes to Mammals: Comprehensive Bioassessment on a Global Scale (652984)
Zacchaeus Compson1, Greg A. C. Singer1, Beverly McClenaghan1, Nicole A. Fahner1, Josh G. Barnes1, Avery McCarthy1 and Mehrdad Hajibabaei2, (1)Centre for Environmental Genomics Applications (CEGA), St. John's, NF, Canada, (2)University of Guelph, Centre for Biodiversity Genomics & Department of Integrative Biology, Guelph, ON, Canada
Designing Environmental DNA Biotic Surveys for the Deep Seafloor (645112)
Olivier Laroche1,2, Oliver Kersten3, Craig R Smith1 and Erica Goetze1, (1)University of Hawaii at Manoa, Department of Oceanography, Honolulu, HI, United States, (2)Institute of Marine Research, Benthic Habitats and Shellfish, Tromso, Norway, (3)University of Oslo, Centre for Ecological and Evolutionary Synthesis, Oslo, Norway
Exploration of eDNA Analysis to Assess Endangered Sea Turtles (655847)
Kirsten Harper1,2, Kelly D. Goodwin2, Lynsey Harper3, Erin LaCasella4, Amy Frey4 and Peter H Dutton4, (1)University of Miami, Cooperative Institute for Marine and Atmospheric Studies, Miami, FL, United States, (2)NOAA Miami, Ocean Chemistry and Ecosystems Division, Atlantic Oceanographic and Meteorological Laboratory, Miami, FL, United States, (3)University of Illinois at Urbana-Champaign, Prairie Research Institute, Champaign, IL, United States, (4)NOAA Southwest Fisheries Science Center, Marine Mammal and Turtle Division, La Jolla, CA, United States
Feeding Habits and Novel Prey of Larval Fishes in the San Francisco Estuary as Revealed by Gut DNA Metabarcoding (655602)
Michelle Jan Jungbluth, San Francisco State University, Estuary & Ocean Science Center, San Francisco, CA, United States and Wim Kimmerer, San Francisco State University - Estuary & Ocean Science Center, Tiburon, CA, United States
Rules of thumb for zooplankton taxonomic assignment using metabarcoding (655369)
Paula Pappalardo1, Kate Hanson2, Allen Collins3, Michael Boyle2 and Karen Osborn4, (1)National Museum of Natural History, Washington, DC, United States, (2)Smithsonian National Museum of Natural History, Washington, DC, United States, (3)Smithsonian National Museum of Natural History, Invertebrate Zoology, Washington, DC, United States, (4)Smithsonian National Museum of Natural History, Invertebrate Zoology, Washington DC, United States
Species Composition and Diversity Patterns across the Salish Sea: Multiple Targeted Metabarcode Analyses of Zooplankton and eDNA in Relation to Chemical Conditions (648723)
Carol A Stepien, NOAA Pacific Marine Environmental Laboratory, Seattle, WA, United States, Julie E Keister, University of Washington Seattle Campus, School of Oceanography, Seattle, WA, United States, Christopher Paight, NOAA Pacific Marine Environmental Laboratory, Ocean Environment Research Division, Seattle, WA, United States, Elizabeth Slikas, University of Washington Seattle Campus, JISAO/NOAA PMEL, Seattle, WA, United States and Emily L Norton, University of Washington, Joint Institute for the Study of the Atmosphere and Ocean, Seattle, WA, United States
Uncovering a Decade of Ecological Change in Monterey Bay Through eDNA Metabarcoding (657082)
Kathleen Pitz, Devon Northcott, Reiko Michisaki, Kristine Walz and Francisco Chavez, Monterey Bay Aquarium Research Institute, Moss Landing, CA, United States