Carbon mineralization in surface and subsurface soils in a subtropical mixed forest in central China

Abstract:

About a half of soil carbon is stored in subsurface soil horizons, their dynamics have the potential to significantly affect carbon balancing in terrestrial ecosystems. However, the main factors regulating subsurface soil carbon mineralization are poorly understood. As affected by mountain humid monsoon, the subtropical mountains in central China has an annual precipitation of about 2000 mm, which causes strong leaching of ions and nutrition. The objectives of this study were to monitor subsurface soil carbon mineralization and to determine if it is affected by nutrient limitation. We collected soil samples (up to 1 m deep) at three locations in a small watershed with three soil layers (0-10 cm, 10-30 cm, below 30 cm). For the three layers, soil organic carbon (SOC) ranged from 35.8 to 94.4 mg g^-1, total nitrogen ranged from 3.51 to 8.03 mg g^-1, microbial biomass carbon (MBC) ranged from 170.6 to 718.4 μg g^-1 soil. We measured carbon mineralization with the addition of N (100 μg N/g soil), P (50 μg P/g soil), and liable carbon (glucose labeled by 5 atom% ¹³C, at five levels: control, 10% MBC, 50% MBC, 100% MBC, 200% MBC). The addition of N and P had negligible effects on CO₂ production in surface soil layers; in the deepest soil layer, the addition of N and P decreased CO₂ production from 4.32 to 3.20 μg C g^-1 soil carbon h^-1. Glucose addition stimulated both surface and subsurface microbial mineralization of SOC, causing priming effects. With the increase of glucose addition rate from 10% to 200% MBC, the primed mineralization rate increased from 0.19 to 3.20 μg C g^-1 soil carbon h^-1 (fifth day of glucose addition). The magnitude of priming effect increased from 28% to 120% as soil layers go deep compare to the basal CO₂ production (fifth day of 200% MBC glucose addition, basal CO₂ production rate for the surface and the deepest soil was 11.17 and 2.88 μg C g^-1 soil carbon h^-1). These results suggested that the mineralization of subsurface carbon is more sensitive to nutrient addition, and carbon mineralization in this layer is likely limited by carbon availability. Thus, any changes in environment conditions (global warming, nitrogen deposition, precipitation pattern change etc.) that affect the distribution of fresh carbon in soil profiles could then stimulate the release of deep soil carbon.