Turn up the lights: Deep-sea in situ application of a high-speed, high-resolution sCMOS camera to observe marine bioluminescence

Brennan Theodore Phillips1, David F Gruber2, John S Sparks3, Ganesh Vasan4, Chris Roman1 and Vincent A Pieribone4, (1)University of Rhode Island Narragansett Bay, Graduate School of Oceanography, Narragansett, RI, United States, (2)City University of New York, Baruch College, New York, NY, United States, (3)American Museum of Natural History, New York, NY, United States, (4)The John B. Pierce Laboratory, New Haven, CT, United States
Abstract:
Observing and measuring marine bioluminescence presents unique challenges in situ. Technology is the greatest limiting factor in this endeavor, with sensitivity, speed and resolution constraining the imaging tools available to researchers. State-of-the-art microscopy cameras offer to bridge this gap. An ultra-low-light, scientific complimentary-metal-oxide-semiconductor (sCMOS) camera was outfitted for in-situ imaging of marine bioluminescence. This system was deployed on multiple deep-sea platforms (manned submersible, remotely operated vehicle, and towed body) in three oceanic regions (Western Tropical Pacific, Eastern Equatorial Pacific, and Northwestern Atlantic) to depths up to 2500m. Using light stimulation, bioluminescent responses were recorded at high frame rates and in high resolution, offering unprecedented low-light imagery of deep-sea bioluminescence in situ. The kinematics and physiology of light production in several zooplankton groups is presented, and luminescent responses at different depths are quantified as intensity vs. time.