A micro-fluidic treadmill for observing suspended plankton in the lab

A significant obstacle to laboratory studies of interactions between small organisms (~ mm) and their fluid environment is our ability to obtain high-resolution images while allowing freedom of motion. This is because as the organisms sink, they will often move out of the field of view of the observation system. One solution to this problem is to impose a water circulation pattern that preserves their location relative to the camera system while imaging the organisms away from the glass walls. To accomplish this we have designed and created a plankton treadmill. Our computer-controlled system consists of a digital video camera attached to a macro or microscope and a micro-fluidic pump whose flow is regulated to maintain a suspended organism’s position relative to the field of view. Organisms are detected and tracked in real time in the video frames, allowing a control algorithm to compensate for any vertical movement by adjusting the flow. The flow control can be manually adjusted using on-screen controls, semi-automatically adjusted to allow the user to select a particular organism to be tracked or fully automatic through the use of classification and tracking algorithms. Experiments with a simple cm-sized cuvette and a number of organisms that are both positively and negatively buoyant have demonstrated the success of the system in permitting longer observation times than would be possible in the absence of a controlled-flow environment. The subjects were observed using a new dual-view, holographic imaging system that provides 3-dimensional microscopic observations with relatively isotropic resolution. We will present the system design, construction, the control algorithm, and some images obtained with the holographic system, demonstrating its effectiveness. Small particles seeded into the flow clearly show the 3D flow fields around the subjects as they freely sink or swim.