Microzooplankton abundance, composition and trophic interactions with phytoplankton and pelagic copepods in the ice-covered and open waters of the Eastern Fram Strait.

Microzooplankton distribution and trophodynamics were examined in the Fram Strait in May and September 2014 as part of the Carbon Bridge multidisciplinary program focused on the biological effects of the Atlantic water transfer to the Arctic Ocean. Samples of microzooplankton were collected along three longitudinal transects from the slope to the ice edge and crossed the Western Spitzbergen Current (WSC). Integrated microzooplankton biomass in the upper 50 m ranged from 0.19 mg C m^-2 above the slope to 3.22 mg C m^-2 within WSC. Mixotrophic oligotrich ciliates from the genus Strombidium comprised most of the biomass and reached their subsurface maximum of 78,000 cells L^-1 and 206 µg C L^-1 in the Atlantic water. This is the highest volumetric biomass of microzooplankton reported from the polar seas so far. Large heterotrophic dinoflagellates Gyrodinium and Protoperidinium were also abundant at several stations. Microzooplankton herbivory rates measured in shipboard dilution experiments ranged from non-detectable within the bloom of Phaeocystis pouchetii to >100% of phytoplankton growth. Overall, the herbivory and production rates were nearly matched and increased from 0.2 d^-1 in May to > 1.0 d^-1 in September. The Atlantic copepod Calanus finmarchicus showed a clear preference for ciliates under the ice and obtained nearly 80% of its carbon-based diet from microzooplankton. These preliminary results support the idea that the microbial food web plays a central role in the marine carbon cycle in the Arctic.