B44A:
Rediscovering Marine Biodiversity: Progress, Promise, and Challenges of Meta-Bar-Coding of Microbes to Mammals II Posters


Session ID#: 28682

Session Description:
Metabarcoding (i.e., large-scale taxonomic identification of complex environmental samples via analysis of orthologous DNA regions) is yielding new insights into biodiversity of marine environments and assemblages from microbes to mammals. The power of high-throughput DNA sequencing allows improved detection of rare, cryptic and novel species, resulting in new global estimates of marine biodiversity and new understanding of the dynamics of marine food webs and ecosystems. There remain both technical and conceptual challenges for metabarcoding analysis and interpretation of these novel results. These include: continued evaluation and comparison of different marker gene regions; deeper resolution and more accurate taxonomic identification of MOTUs (Molecular Operational Taxonomic Units); and more attention to validation and ground-truthing of metabarcoding results. Ongoing research is demonstrating prospects for broader application of metabarcoding, including quantification of taxon abundance or biomass, examination of trophic interactions and food-web dynamics, remote detection of marine organisms based on eDNA (dissolved, extracellular environmental DNA), and use in time-series analysis and ecosystem monitoring.
Primary Chair:  Ann C Bucklin, University of Connecticut, Marine Sciences, Groton, CT, United States
Co-chairs:  Leocadio Blanco-Bercial, Bermuda Institute of Ocean Sciences, St. George's, Bermuda and Ryuji Machida, Academia Sinica, Biodiversity Research Center, Taipei, Taiwan
Moderators:  Ann C Bucklin, University of Connecticut, Marine Sciences, Groton, CT, United States and Ryuji Machida, Academia Sinica, Biodiversity Research Center, Taipei, Taiwan
Student Paper Review Liaison:  Leocadio Blanco-Bercial, Bermuda Institute of Ocean Sciences, St. George's, Bermuda
Index Terms:

4817 Food webs, structure, and dynamics [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
4840 Microbiology and microbial ecology [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
4855 Phytoplankton [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
4890 Zooplankton [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
Cross-Topics:
  • MM - Microbiology and Molecular Ecology

Abstracts Submitted to this Session:

Inside Out – Shedding Light on the Endobiont Bacterial Community of Foraminifera (316988)
Iines Salonen1, Myrsini Chronopoulou1, Elina Leskinen1, Clare Bird2, Gert-Jan Reichart3,4 and Karoliina Annika Koho1, (1)University of Helsinki, Helsinki, Finland, (2)University of Edinburgh, Edinburgh, United Kingdom, (3)Royal Netherlands Institute for Sea Research, Den Burg, Netherlands, (4)Utrecht University, Utrecht, Netherlands
Metagenetic analysis of temporal changes in diet composition of the copepod Calanus finmarchicus in the Gulf of Maine (323891)
Heidi Danielle Yeh, University of Connecticut - Avery Point Campus, Uncasville, CT, United States, Jennifer M Questel, University of Connecticut, Department of Marine Sciences, Groton, CT, United States and Ann C Bucklin, University of Connecticut, Marine Sciences, Groton, CT, United States
Seasonal Variability in the Persistence of Dissolved Environmental DNA (eDNA) in a Marine System: The Role of Microbial Nutrient Limitation. (317984)
Ian Salter, Laboratoire d'Océanographie Microbienne, Argir, Faroe Islands
Zooplankton diversity of the NW Atlantic continental shelf based on 18S rRNA metabarcodes (320537)
Ann C Bucklin, University of Connecticut, Marine Sciences, Groton, CT, United States, Jennifer M Questel, University of Connecticut, Department of Marine Sciences, Groton, CT, United States, Nancy J Copley, Woods Hole Oceanographic Inst, Woods Hole, MA, United States and Peter H Wiebe, Woods Hole Oceanographic Institution, Woods Hole, MA, United States
New Insights into the Phytoplankton Community at Helgoland Roads Using Next Generation Sequencing with Focus on Harmful Algae (314282)
Laura Käse1, Katja Metfies2, Karen Wiltshire3, Maarten Boersma1, Bernhard Fuchs4 and Alexandra Kraberg1, (1)Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Biologische Anstalt Helgoland, Helgoland, Germany, (2)Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven, Germany, (3)Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, List/Sylt, Germany, (4)Max-Planck-Institute for Marine Microbiology, Molecular Ecology, Bremen, Germany
Evaluation of marine zooplankton community structure through environmental DNA metabarcoding (324141)
Anni Djurhuus1, Kathleen Pitz2, Natalie A Sawaya3, Jaimie Rojas-Márquez4, Brianna Michaud3, Enrique Montes3, Frank Muller-Karger5 and Mya Breitbart3, (1)University of South Florida, College of Marine Science, St Petersburg, FL, United States, (2)Monterey Bay Aquatic Research Institute, United States, (3)University of South Florida, United States, (4)3., Fundación La Salle de Ciencias Naturales, Estacion de Investigaciones Marinas, Isla de Margarita, Venezuela, (5)University of South Florida, St. Petersburg, FL, United States
Beyond Biodiversity: Metabarcoding As A Tool Of Ecological Exploration (302601)
Luke McKane Stewart1, Leocadio Blanco-Bercial2 and Amy E. Maas2, (1)Bermuda Institute of Ocean Sciences, St. Georges, Bermuda, (2)Bermuda Institute of Ocean Sciences, St. George's, Bermuda
Assessment of Proximate, Lipid Profile and Molecular Characterization of Green Microalgae Isolated from South-West Nigeria (303700)
Sandra Chinwendu Akagha1, Kedong Yin2 and Dike Ikegwu Nwankwo1, (1)University of Lagos, Department of Marine Sciences, Lagos, Nigeria, (2)Sun Yat-Sen University, School of Marine Science, Guangzhou, China
Metabarcoding analysis of Zooplankton biodiversity in the southern region of Gulf of Mexico (312761)
Paola G. Batta-Lona1, Clara E Galindo-Sanchez2, Maria C Arteaga,3, Sylvia P.A. Jiménez-Rosenberg4, Jaime Gasca3, Anaid Saavedra-Flores3, Javier Robles-Flores3, Miguel Martinez3 and Clara Hereu3, (1)Centro de Investigación Científica y de Educación Superior de Ensenada, Marine Biotechnology, Ensenada, Mexico, (2)Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada, Mexico, (3)Center for Scientific Research and Higher Education at Ensenada, Ensenada, Mexico, (4)Instituto Politecnico Nacional - CICIMAR, Mexico
Mechanisms of Biodiversity Formation: Insights from Molecular Genetic Studies of Planktonic Shrimps in the Indo-Pacific Region (325825)
Mary Mar Padohinog Noblezada, University of the Philippines Visayas, Institute of Marine Fisheries and Oceanology, College of Fisheries & Ocean Sciences, Iloilo City, Philippines, Wilfredo L. Campos, University of the Philippines Visayas, Division of Biological Sciences, College of Arts and Sciences, Iloilo City, Philippines, Hiroomi Miyamoto, Tohoku National Fisheries Research Institute, Japan Fisheries Research and Education Agency, Japan, Fatimah Yusoff, Institute of Bioscience,, Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia and Nishida Shuhei, Atmosphere and Ocean Research Institute University of Tokyo, Tokyo, Japan
Metabarcoding Arctic zooplankton: Toward a region-specific reference DNA sequence database (320893)
Hayley Mills DeHart, University of Connecticut, Department of Marine Science, Groton, CT, United States, Jennifer M Questel, University of Connecticut, Department of Marine Sciences, Groton, CT, United States, Russell R Hopcroft, Univ Alaska, Fairbanks, AK, United States, Ann C Bucklin, University of Connecticut, Marine Sciences, Groton, CT, United States and Ksenia N. Kosobokova, Russian Academy of Sciences, P.P. Shirsov Institute of Oceanology, Moscow, Russia
Comparing the Characterization of Coastal Marine Bacterioplankton Population Dynamics and Community Ecology by Four 16S Ribosomal RNA Primer Sets (303388)
Emma Wear1, Elizabeth Wilbanks1, Craig Nelson2 and Craig A Carlson3, (1)University of California Santa Barbara, Santa Barbara, CA, United States, (2)University of Hawaii at Manoa, School of Ocean, Earth Science, and Technology, Honolulu, HI, United States, (3)University of California Santa Barbara, Marine Science Institute/Department of Ecology, Evolution and Marine Biology, Santa Barbara, CA, United States
Comparative Spatiotemporal Analysis of Microbial Communities in the San Francisco Bay-Delta with two 16S rRNA Primer sets (322858)
Anna Nichole Rasmussen1, Julian Damashek1,2, Emiley Eloe-Fadrosh3 and Christopher Francis1, (1)Stanford University, Earth System Science, Stanford, CA, United States, (2)University of Georgia, Marine Science, Athens, GA, United States, (3)Joint Genome Institute, Walnut Creek, CA, United States
Mesoscale Eddies Modulate Phytoplankton Biogeography in the California Current. (321970)
Erin Horn1, Sophie Clayton1, Mohammad Dehghani Ashkezari2, Rachelle Lim1 and E. Virginia Armbrust1, (1)University of Washington, School of Oceanography, Seattle, WA, United States, (2)Massachusetts Institute of Technology, Department of Earth, Atmospheric and Planetary Sciences, Cambridge, MA, United States

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