B002:
Rediscovering marine biodiversity: progress, promise, and challenges of metabarcoding of microbes to mammals


Session ID#: 28682

Session Description:
Metabarcoding (i.e., large-scale taxonomic identification of complex environmental samples via analysis of orthologous DNA regions) is yielding new insights into biodiversity of marine environments and assemblages from microbes to mammals. The power of high-throughput DNA sequencing allows improved detection of rare, cryptic and novel species, resulting in new global estimates of marine biodiversity and new understanding of the dynamics of marine food webs and ecosystems. There remain both technical and conceptual challenges for metabarcoding analysis and interpretation of these novel results. These include: continued evaluation and comparison of different marker gene regions; deeper resolution and more accurate taxonomic identification of MOTUs (Molecular Operational Taxonomic Units); and more attention to validation and ground-truthing of metabarcoding results.  Ongoing research is demonstrating prospects for broader application of metabarcoding, including quantification of taxon abundance or biomass, examination of trophic interactions and food-web dynamics, remote detection of marine organisms based on eDNA (dissolved, extracellular environmental DNA), and use in time-series analysis and ecosystem monitoring.
Primary Chair:  Ann C Bucklin, University of Connecticut, Marine Sciences, Groton, CT, United States
Co-chairs:  Leocadio Blanco-Bercial, Bermuda Institute of Ocean Sciences, St. George's, Bermuda and Ryuji Machida, Academia Sinica, Biodiversity Research Center, Taipei, Taiwan
Index Terms:

4817 Food webs, structure, and dynamics [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
4840 Microbiology and microbial ecology [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
4855 Phytoplankton [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
4890 Zooplankton [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
Cross-Topics:
  • BE - Benthic Ecosystems
    *Use ME: Marine Ecosystems*
  • MM - Microbiology and Molecular Ecology
  • PE - Pelagic Ecosystems
    *Use ME: Marine Ecosystems*

Abstracts Submitted to this Session:

Luke McKane Stewart1, Leocadio Blanco-Bercial2 and Amy E. Maas2, (1)Bermuda Institute of Ocean Sciences, St. Georges, Bermuda, (2)Bermuda Institute of Ocean Sciences, St. George's, Bermuda
Elizabeth Andruszkiewicz, Stanford University, Portola Valley, CA, United States, Alexandria B Boehm, Stanford University, Stanford, CA, United States and Lauren Sassoubre, University at Buffalo, Buffalo, NY, United States
Emma Wear1, Elizabeth Wilbanks1, Craig Nelson2 and Craig A Carlson3, (1)University of California Santa Barbara, Santa Barbara, CA, United States, (2)University of Hawaii at Manoa, School of Ocean, Earth Science, and Technology, Honolulu, HI, United States, (3)University of California Santa Barbara, Marine Science Institute/Department of Ecology, Evolution and Marine Biology, Santa Barbara, CA, United States
Sandra Chinwendu Akagha1, Kedong Yin2 and Dike Ikegwu Nwankwo1, (1)University of Lagos, Department of Marine Sciences, Lagos, Nigeria, (2)Sun Yat-Sen University, School of Marine Science, Guangzhou, China
Holly Bik1, Taruna Schuelke2, Tiago Jose Pereira2 and Sarah M Hardy3, (1)University of California Riverside, Department of Nematology, Riverside, CA, United States, (2)University of California, Riverside, Department of Nematology, Riverside, CA, United States, (3)University of Alaska Fairbanks, Fairbanks, AK, United States
Nathan Geraldi, King Abdullah University of Science and Technology (KAUST), Red Sea Research Center (RSRC), Thuwal, Saudi Arabia and Carlos M Duarte, King Abdullah University of Science and Technology, Red Sea Research Center, Thuwal-Jeddah 23955-6900, Saudi Arabia
Ryuji Machida, Academia Sinica, Biodiversity Research Center, Taipei, Taiwan, Matthieu Leray, Smithsonian Tropical Research Institute, Panama, Shian-Lei Ho, Academia Sinica, Biodiversity Research Centre, Taiwan and Nancy Knowlton, Sant Chair for Marine Science, Smithsonian’s National Museum of Natural History, Washington, DC, United States
Nobuharu Inaba, National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, Yokohama, Japan
Leocadio Blanco-Bercial1, Tim J Noyes1, Divya Sirdeshpande2, Luca Peruzza3, Maria Granquist2 and Jonas Schroder1, (1)Bermuda Institute of Ocean Sciences, St. George's, Bermuda, (2)Lehigh University, PA, United States, (3)National Oceanography Centre, Southampton, United Kingdom
Sara Zamora-Terol, Stockholm University, Department of Ecology, Environment and Plant Science, Stockholm, Sweden, Andreas Novotny, Stockholm University, Department of Ecology, Environment and Plant Science, Sweden and Monika Winder, Stockholm University, Department of Ecology, Environment and Plant Sciences, Stockholm, Sweden
Paola Batta-Lona1, Clara E Galindo-Sanchez2, Maria C Arteaga,3, Sylvia P.A. Jiménez-Rosenberg4, Jaime Gasca3, Anaid Saavedra-Flores3, Javier Robles-Flores3, Miguel Martinez3 and Clara Hereu3, (1)Centro de Investigación Científica y de Educación Superior de Ensenada, Marine Biotechnology, Ensenada, Mexico, (2)Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada, Mexico, (3)Center for Scientific Research and Higher Education at Ensenada, Ensenada, Mexico, (4)Instituto Politecnico Nacional - CICIMAR, Mexico
Carol A Stepien1, Anna E. Elz2, Matthew D. Snyder2 and Nathaniel T. Marshall2, (1)NOAA Pacific Marine Environmental Laboratory, Seattle, WA, United States, (2)NOAA Pacific Marine Environmental Laboratory, G3-Genetics and Genomics Group, Seattle, WA, United States
Laura Käse1, Katja Metfies2, Karen Wiltshire3, Maarten Boersma1, Bernhard Fuchs4 and Alexandra Kraberg1, (1)Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Biologische Anstalt Helgoland, Helgoland, Germany, (2)Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven, Germany, (3)Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, List/Sylt, Germany, (4)Max-Planck-Institute for Marine Microbiology, Molecular Ecology, Bremen, Germany
Lisa Nigro1, Klaudia Hernandez2, Beatriz Yannicelli3, Nelson Silva4 and Wade H Jeffrey1, (1)University of West Florida, Center for Environmental Diagnostics and Bioremediation, Pensacola, FL, United States, (2)Universidad Andres Bello, Departamento de Ecología y Biodiversidad, Viña del Mar, Chile, (3)Universidad Católica del Norte, Ecology and Sustainable Management of Oceanic Islands (ESMOI), Coquimbo, Chile, (4)Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile
Jordan Paul Wilson1, Amy Apprill2, Carolyn Miller3, John W Durban4, Matthew Leslie5, Gustavo Chiang6, Paulina Bahamonde6, Holly Fearnbach7 and Lance Barrett-Lennard8, (1)NOAA Ernest F Hollings Undergraduate Scholarship Program, West Sacramento, CA, United States, (2)Woods Hole Oceanographic Institution, Department of Marine Chemistry & Geochemistry, Woods Hole, MA, United States, (3)Woods Hole Oceanographic Institution, Woods Hole, MA, United States, (4)National Oceanic and Atmospheric Administration - National Marine Fisheries Service, Southwest Fisheries Science Center, La Jolla, CA, United States, (5)Smithsonian Institute, DC, United States, (6)Fundación Meri, Santiago, Chile, (7)SR3 Sealife Response, Rehabilitation, and Research, WA, United States, (8)Coastal Ocean Research Institute, Vancouver Aquarium, Vancouver, BC and Zoology Department, University of British Columbia, BC, Canada
Iines Salonen1, Myrsini Chronopoulou1, Elina Leskinen1, Clare Bird2, Gert-Jan Reichart3,4 and Karoliina Annika Koho1, (1)University of Helsinki, Helsinki, Finland, (2)University of Edinburgh, Edinburgh, United Kingdom, (3)Royal Netherlands Institute for Sea Research, Den Burg, Netherlands, (4)Utrecht University, Utrecht, Netherlands
Ian Salter, Laboratoire d'Océanographie Microbienne, Argir, Faroe Islands
Eric D Salomaki, University of Rhode Island, Cell and Molecular Biology, Kingston, RI, United States and Bethany D. Jenkins, University of Rhode Island, Cell and Molecular Biology and Graduate School of Oceanography, Narragansett, RI, United States
Ann C Bucklin, University of Connecticut, Marine Sciences, Groton, CT, United States, Jennifer M Questel, University of Connecticut, Department of Marine Sciences, Groton, CT, United States, Nancy J Copley, Woods Hole Oceanographic Inst, Woods Hole, MA, United States and Peter H Wiebe, Woods Hole Oceanographic Institution, Woods Hole, MA, United States
Hayley Mills DeHart, University of Connecticut, Department of Marine Science, Groton, CT, United States, Jennifer M Questel, University of Connecticut, Department of Marine Sciences, Groton, CT, United States, Russell R Hopcroft, Univ Alaska, Fairbanks, AK, United States and Ann C Bucklin, University of Connecticut, Marine Sciences, Groton, CT, United States
Juan Ramon Beltran Castro1, Anidia Blanco Jarvio2, Jaime Gomez Gutierrez1 and Sergio Hernández Trujillo1, (1)Organization Not Listed, PLANKTON DEPARTAMENT, La Paz, Mexico, (2)UABC Autonomous University of Baja California Sur, DEPARTAMENTO DE PESQUERIAS, La Paz, Mexico
Anna Nichole Rasmussen1, Julian Damashek1,2, Emiley Eloe-Fadrosh3 and Christopher Francis1, (1)Stanford University, Earth System Science, Stanford, CA, United States, (2)University of Georgia, Marine Science, Athens, GA, United States, (3)Joint Genome Institute, Walnut Creek, CA, United States
Heidi Danielle Yeh, University of Connecticut - Avery Point Campus, Uncasville, CT, United States, Jennifer M Questel, University of Connecticut, Department of Marine Sciences, Groton, CT, United States and Ann C Bucklin, University of Connecticut, Marine Sciences, Groton, CT, United States
Anni Djurhuus1, Kathleen Pitz2, Natalie A Sawaya3, Jaimie Rojas-Márquez4, Brianna Michaud3, Enrique Montes3, Frank Muller-Karger3 and Mya Breitbart3, (1)University of South Florida, College of Marine Science, St Petersburg, FL, United States, (2)Monterey Bay Aquatic Research Institute, United States, (3)University of South Florida, United States, (4)3., Fundación La Salle de Ciencias Naturales, Estacion de Investigaciones Marinas, Isla de Margarita, Venezuela
Matthieu Leray, Smithsonian Tropical Research Institute, Panama
Mary Mar Padohinog Noblezada, University of the Philippines Visayas, Institute of Marine Fisheries and Oceanology, College of Fisheries & Ocean Sciences, Iloilo City, Philippines, Wilfredo L. Campos, University of the Philippines Visayas, Division of Biological Sciences, College of Arts and Sciences, Iloilo City, Philippines, Hiroomi Miyamoto, Tohoku National Fisheries Research Institute, Japan Fisheries Research and Education Agency, Japan, Fatimah Yusoff, Institute of Bioscience,, Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia and Nishida Shuhei, Atmosphere and Ocean Research Institute University of Tokyo, Tokyo, Japan

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