B41A:
Rediscovering Marine Biodiversity: Progress, Promise, and Challenges of Meta-Bar-Coding of Microbes to Mammals I


Session ID#: 36916

Session Description:
Metabarcoding (i.e., large-scale taxonomic identification of complex environmental samples via analysis of orthologous DNA regions) is yielding new insights into biodiversity of marine environments and assemblages from microbes to mammals. The power of high-throughput DNA sequencing allows improved detection of rare, cryptic and novel species, resulting in new global estimates of marine biodiversity and new understanding of the dynamics of marine food webs and ecosystems. There remain both technical and conceptual challenges for metabarcoding analysis and interpretation of these novel results. These include: continued evaluation and comparison of different marker gene regions; deeper resolution and more accurate taxonomic identification of MOTUs (Molecular Operational Taxonomic Units); and more attention to validation and ground-truthing of metabarcoding results. Ongoing research is demonstrating prospects for broader application of metabarcoding, including quantification of taxon abundance or biomass, examination of trophic interactions and food-web dynamics, remote detection of marine organisms based on eDNA (dissolved, extracellular environmental DNA), and use in time-series analysis and ecosystem monitoring.
Primary Chair:  Ann C Bucklin, University of Connecticut, Marine Sciences, Groton, CT, United States
Co-chairs:  Leocadio Blanco-Bercial, Bermuda Institute of Ocean Sciences, St. George's, Bermuda and Ryuji Machida, Academia Sinica, Biodiversity Research Center, Taipei, Taiwan
Moderators:  Leocadio Blanco-Bercial, Bermuda Institute of Ocean Sciences, St. George's, Bermuda and Ryuji Machida, Academia Sinica, Biodiversity Research Center, Taipei, Taiwan
Student Paper Review Liaison:  Ann C Bucklin, University of Connecticut, Marine Sciences, Groton, CT, United States
Index Terms:

4817 Food webs, structure, and dynamics [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
4840 Microbiology and microbial ecology [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
4855 Phytoplankton [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
4890 Zooplankton [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
Cross-Topics:
  • MM - Microbiology and Molecular Ecology

Abstracts Submitted to this Session:

Nematode microbiome assemblages do not correlate with host phylogeny, geographic region or feeding morphology in marine sediment habitats (303806)
Holly Bik1, Taruna Schuelke2, Tiago Jose Pereira2 and Sarah M Hardy3, (1)University of California Riverside, Department of Nematology, Riverside, CA, United States, (2)University of California, Riverside, Department of Nematology, Riverside, CA, United States, (3)University of Alaska Fairbanks, Fairbanks, AK, United States
Global diversity of marine metazoans based on metagenomes. (305377)
Nathan Geraldi, King Abdullah University of Science and Technology (KAUST), Red Sea Research Center (RSRC), Thuwal, Saudi Arabia and Carlos M Duarte, King Abdullah University of Science and Technology, Red Sea Research Center, Thuwal-Jeddah 23955-6900, Saudi Arabia
Introducing MIDORI: metazoan mitochondrial gene sequence reference datasets for taxonomic assignment of environmental samples (309772)
Ryuji Machida, Academia Sinica, Biodiversity Research Center, Taipei, Taiwan, Matthieu Leray, Smithsonian Tropical Research Institute, Panama, Shian-Lei Ho, Academia Sinica, Biodiversity Research Centre, Taiwan and Nancy Knowlton, Sant Chair for Marine Science, Smithsonian’s National Museum of Natural History, Washington, DC, United States
Untangling Trophic Interactions in Marine Plankton Communities through DNA barcoding approaches (312383)
Sara Zamora-Terol, Stockholm University, Department of Ecology, Environment and Plant Science, Stockholm, Sweden, Andreas Novotny, Stockholm University, Department of Ecology, Environment and Plant Science, Sweden and Monika Winder, Stockholm University, Department of Ecology, Environment and Plant Sciences, Stockholm, Sweden
Targeted Metagenomic Assays to Understand Marine Community Responses in Species Composition, Diversity, and Population Genetics (313654)
Carol A Stepien1, Anna E. Elz2, Matthew D. Snyder2 and Nathaniel T. Marshall2, (1)NOAA Pacific Marine Environmental Laboratory, Seattle, WA, United States, (2)NOAA Pacific Marine Environmental Laboratory, G3-Genetics and Genomics Group, Seattle, WA, United States
16S rRNA Gene Based Microbial Community Composition Analysis and Productivity Patterns in a Transect From the Humboldt Current System to Islands of the South Pacific Gyre (314486)
Lisa Nigro1, Klaudia Hernandez2, Beatriz Yannicelli3, Nelson Silva4 and Wade H Jeffrey1, (1)University of West Florida, Center for Environmental Diagnostics and Bioremediation, Pensacola, FL, United States, (2)Universidad Andres Bello, Departamento de Ecología y Biodiversidad, Viña del Mar, Chile, (3)Universidad Católica del Norte, Ecology and Sustainable Management of Oceanic Islands (ESMOI), Coquimbo, Chile, (4)Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile

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