MM44C:
Proteomics and Lipidomics: Expanding the Macromolecular Toolbox to Understand Oceanic Processes


Session ID#: 9504

Session Description:
Analysis of proteins and lipids produced by an organism can be a direct view into adaptation strategies used by an individual or community in response to changing environmental conditions.  In addition to dynamic cellular responses of lipids and proteins to environmental perturbations, these two classes of organic molecules can also be preserved long after death of the organism.  Thus, the analysis of proteins and lipids can either provide real-time biological interaction evidence, or when examined in aged matrices, they can be used as tracers to reconstruct past environments.  Discovery of these analytes in a variety of locations and matrices (e.g., sediments, frustules, dissolved organic matter fractions) emphasizes the great potential for expanding our understanding of ecosystems using proteomic and lipidomic methodologies.  We invite contributions from all studies examining the use of marine protein and lipids to elucidate biological, geological or physical systems in the ocean. We encourage studies that integrate multiple “omics” approaches and  aim for this session to open a discussion on relationships between transcripts, proteins, lipids, and their resulting metabolites or activities so that integration and interrogation of these analyses can provide novel biological insight into marine systems.
Primary Chair:  Elisha K. Moore, Royal Netherlands Institute for Sea Research, Den Burg, 1790, Netherlands; Rutgers University, Marine and Coastal Sciences, New Brunswick, NJ, United States
Chairs:  Brook L Nunn, University of Washington, Department of Genome Sciences, Seattle, United States and H. Rodger Harvey, Old Dominion University, Ocean, Earth and Atmospheric Sciences, Norfolk, VA, United States
Moderators:  Brook L Nunn, University of Washington, Department of Genome Sciences, Seattle, United States and H. Rodger Harvey, Old Dominion University, Ocean, Earth and Atmospheric Sciences, Norfolk, VA, United States
Student Paper Review Liaisons:  Elisha K. Moore, Royal Netherlands Institute for Sea Research, Den Burg, 1790, Netherlands and Brook L Nunn, University of Washington, Department of Genome Sciences, Seattle, United States
Index Terms:

1694 Instruments and techniques [GLOBAL CHANGE]
4803 Analytical chemistry [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
4805 Biogeochemical cycles, processes, and modeling [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
4840 Microbiology and microbial ecology [OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL]
Co-Sponsor(s):
  • B - Biogeochemistry and Nutrients
  • HE - High Latitude Environments
  • IS - Instrumentation & Sensing Technologies
  • PP - Phytoplankton and Primary Production

Abstracts Submitted to this Session:

 
MM44C-0500
Heat stress dictates microbial lipid composition in hydrothermal marine sediments (92068)
Miriam Sollich1, Marcos Y Yoshinaga2, Stefan Häusler3, Kai-Uwe Hinrichs4 and Solveig I. Bühring2, (1)Marum Institute, University of Bremen, Hydrothermal Geomicrobiology, Bremen, Germany, (2)MARUM - University of Bremen, Bremen, Germany, (3)Max-Planck-Institute, Bremen, Germany, (4)MARUM / University of Bremen, Bremen, Germany
 
MM44C-0502
Microbial contributions to suspended POM accumulation in an ultraoligotrophic water column, investigated using intact polar diacyglycerol biomarker lipids (88294)
Jenan J Kharbush1, Andrew E Allen2, Ahmed Moustafa3, Pieter Dorrestein4 and Lihini Aluwihare1, (1)Scripps Institution of Oceanography, La Jolla, CA, United States, (2)University of California, San Diego / J Craig Venter Institute, Scripps Institution of Oceanography, La Jolla, CA, United States, (3)The American University in Cairo, (4)University of California San Diego, Collaborative Mass Spectrometry Innovation Center, La Jolla, CA, United States
 
MM44C-0503
New insights into the role of phosphate-free lipids in anaerobic environments (91848)
Florence Schubotz1, Ana De Santiago Torio2, Jennifer Kuehl3, Carly Grant4, Lilah Rahn-Lee4, Adam P Arkin3,4, Adam M Deutschbauer3, Roger E Summons5 and Tanja Bosak2, (1)MARUM - University of Bremen, Bremen, Germany, (2)Massachusetts Institute of Technology, Cambridge, MA, United States, (3)Lawrence Berkeley National Laboratory, Berkeley, CA, United States, (4)University of California, Berkeley, (5)MIT Lincoln Laboratory, Lexington, MA, United States
 
MM44C-0504
Tracking Intact Phospholipids and Triacylglycerides in Bering Sea Euphausiids during Two Pulsed Feeding Experiments via Tandem LC-MS (92641)
Rachel L Pleuthner, Old Dominion University, Norfolk, VA, United States and H. Rodger Harvey, Old Dominion University, Ocean, Earth and Atmospheric Sciences, Norfolk, VA, United States
 
MM44C-0505
Methods for and Insights from Phosphoproteome Analysis in Marine Microbes (91972)
Noelle Adriana Held1,2, Mak A Saito2 and Matthew McIlvin2, (1)Massachusetts Institute of Technology, Cambridge, MA, United States, (2)Woods Hole Oceanographic Institution, Woods Hole, MA, United States
 
MM44C-0506
Linking Ecological, Environmental and Biogeochemical Data with Multi'omics Analysis (88629)
Harald Hasler-Sheetal1, Max CN Castorani2, Lena Fragner3, Yonghui Zeng1, Marianne Holmer4, Ronnie N Glud1, Wolfram Weckwerth3 and Donald E Canfield1, (1)University of Southern Denmark, Nordic Center of Earth Evolution, Department of Biology, Odense, Denmark, (2)University of California, Marine Science Institute, Santa Barbara, CA, United States, (3)University of Vienna, MOSYS, Vienna, Austria, (4)University of Southern Denmark, Biology, Odense, Denmark
 
MM44C-0507
The peptide equivalent of the 16SrRNA assay: Revealing Phylogeny and Function (90374)
Brook L Nunn1, Damon Hutton May2, Emma Timmins-Schiffman2, Molly Mikan3, H. Rodger Harvey3 and William S Noble2, (1)University of Washington, Department of Genome Sciences, Seattle, United States, (2)University of Washington, Department of Genome Sciences, Seattle, WA, United States, (3)Old Dominion University, Norfolk, VA, United States
 
MM44C-0508
Intracellular carbon isotope distributions of continuous-culture Allochromatium vinosum grown on acetate vs. CO2. (88859)
Tiantian Tang1, Wiebke Mohr2, Sarah Sattin3, Daniel Rogers3, Peter R Girguis3 and Ann Pearson4, (1)Xiamen University, Xiamen, China, (2)Max Planck Institute for Marine Microbiology, Biogeochemistry, Bremen, Germany, (3)Harvard University, Cambridge, MA, United States, (4)Harvard University, Department of Earth and Planetary Sciences, Cambridge, MA, United States
 
MM44C-0509
Needles in the Blue Sea: Sub-species Specificity by Targeted Metaproteomics of the Vast Oceanic Microbial Metaproteome (92099)
Matthew McIlvin and Mak A Saito, Woods Hole Oceanographic Institution, Woods Hole, MA, United States
 
MM44C-0510
Achieving high confidence protein annotations in a sea of unknowns (89772)
Emma Timmins-Schiffman1, Damon Hutton May1, William S Noble1, Brook L Nunn2, Molly Mikan3 and H. Rodger Harvey3, (1)University of Washington, Department of Genome Sciences, Seattle, WA, United States, (2)University of Washington, Department of Genome Sciences, Seattle, United States, (3)Old Dominion University, Norfolk, VA, United States
 
MM44C-0511
Plastid proteomics for elucidating iron limited remodeling of plastid physiology in diatoms (93356)
Kristofer M Gomes, University of Rhode Island, Cell and Molecular Biology, Kingston, RI, United States, Brook L Nunn, University of Washington, Department of Genome Sciences, Seattle, United States and Bethany D. Jenkins, University of Rhode Island, Cell and Molecular Biology and Graduate School of Oceanography, Narragansett, RI, United States
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